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<a href="/article.html">技術(shù)文章</a> > 小鼠的灌胃給藥技術(shù)指導(dǎo)</span> </div> <div id="vpovlqsclme" class="right_content"> <div id="vpovlqsclme" class="art-cont"> <h1 class="art-title">小鼠的灌胃給藥技術(shù)指導(dǎo)</h1> <div id="vpovlqsclme" class="art-info"> 發(fā)布時(shí)間： 2022-05-25　　點(diǎn)擊次數(shù)： 697次 </div> <div id="vpovlqsclme" class="art-body"> <div>　　小鼠的灌胃給藥技術(shù)指導(dǎo)</div><div>　　從新藥研發(fā)角度來(lái)說(shuō)，因口服給藥是方便、經(jīng)濟(jì)安全的方式，在研究中較常用。動(dòng)物實(shí)驗(yàn)中，灌胃是口服給藥常用的方法，然而，看似簡(jiǎn)單的幾步，可對(duì)于初學(xué)者常常因?yàn)槭址ú划?dāng)而傷到氣道或食道，進(jìn)而引起小鼠死或者逐漸消瘦至死亡。那么在灌胃的實(shí)驗(yàn)中，我們究竟該掌握哪些注射手法和技巧呢？下面一起來(lái)看看！</div><div>　　灌胃：借助器械（灌胃針）將藥物直接灌入動(dòng)物胃內(nèi)；</div><div>　　藥物循環(huán)途徑：食管→胃→小腸→小腸毛細(xì)血管（吸收入血）→空回腸靜脈→腸系膜上靜脈→肝門靜脈→肝→肝靜脈→下腔靜脈→右心房→右心室的吸收進(jìn)入血液循環(huán)。</div><div>　　特點(diǎn)：</div><div>　　安全性高：各種給藥方式中，口服給藥是安全的；</div><div>　　可選的劑型比較多：只要保證藥物是均勻且穩(wěn)定即可；</div><div>　　給藥體積大：小鼠：0.1-0.8ml（0.2 ml /10g）；大鼠：1-3ml。</div><div>　　生物利用度低：藥物經(jīng)胃腸道吸收，并存在胃腸道首過(guò)代謝和肝臟首過(guò)代謝，生物利用率相對(duì)較低，僅為靜脈的25-30%；對(duì)于部分化合物吸收不恒定，誤差較大。</div><div>　　灌胃的實(shí)驗(yàn)步驟</div><div>　　一、準(zhǔn)備工具</div><div>　　8/9/12號(hào)灌胃針頭配套1.0 ml注射器；如下圖為9號(hào)灌胃針頭/1.0 ml注射器，為了防止插入時(shí)造成損傷，針頭呈倒水滴型。</div><div>　　號(hào)灌胃針頭/1.0ml注射器</div><div>　　注：</div><div>　　灌胃針的分類：如下圖為小鼠灌胃常見(jiàn)灌胃針類別，根據(jù)尺寸不同有8/9/12號(hào)；另外還有直頭和彎頭區(qū)別；</div><div>　　灌胃針的選擇：尺寸大小的選擇一般可根據(jù)小鼠的年幼決定，如＜30g，建議用8、9號(hào)；30g以上可選擇12號(hào)；其中9號(hào)針頭直徑小于常規(guī)的大頭，特別適用幼鼠；直頭彎頭可根據(jù)個(gè)人習(xí)慣選擇，初學(xué)者建議先適用彎頭練習(xí)；</div><div>　　灌胃針的獲取：可直接購(gòu)買，不建議手動(dòng)處理的注射器針頭，易刺傷小鼠食道；</div><div>　　灌胃針的深度：因?yàn)閯?dòng)物有個(gè)體差異，實(shí)驗(yàn)前可先測(cè)量小鼠口腔至胃的距離，進(jìn)而選擇合適長(zhǎng)度的灌胃針，避免插入過(guò)深或過(guò)淺。小鼠入針一般3-4cm，大鼠一般4-6cm。</div><div>　　二、操作步驟</div><div>　　固定小鼠：用左手拇指和食指抓住鼠耳和頸部皮膚，用左手的無(wú)名指和小指夾其背部皮膚和尾部，使其頭、頸和身體在一條直線；若第一次抓取時(shí)不得當(dāng)，一定要放開(kāi)重抓，以免影響后續(xù)操作；</div><div>　　進(jìn)針：右手持注射器（1ml的注射器配灌胃針頭），從一側(cè)嘴角進(jìn)針，輕壓舌根，輕輕向前推，進(jìn)入食管后會(huì)感覺(jué)有個(gè)刺空感，緩慢推進(jìn)，一般當(dāng)針插入小鼠3～4cm，即灌胃針的3/4時(shí)即可注射；</div><div>　　注意：若感到阻力或動(dòng)物掙扎比較厲害時(shí)，說(shuō)明插錯(cuò)位置，應(yīng)馬上停止進(jìn)針或?qū)⑨槹纬觯淮齽?dòng)物恢復(fù)平靜后，再次嘗試。</div> </div> <div id="vpovlqsclme" class="art-next"> <dl 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